Liquid culture is a suspension of mycelium in a nutrient medium, designed for fast and reliable inoculation. Unlike spore prints or spore syringes, liquid culture provides active, live mycelium, which results in quicker colonization times and more successful growth. Whether you're a hobbyist or a commercial grower, our liquid cultures help you start your projects on the right foot.
Liquid Culture Syringe
$18.75Price
How to Use Liquid Culture Syringes:
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Preparation:
- Before using your liquid culture syringe, make sure all tools and surfaces are clean and sanitized to prevent contamination.
- You may want to work in a clean environment such as a still air box, glove box, or under a laminar flow hood for optimal sterility.
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Inoculating Grain or Substrate:
- Sterilize your grain jars or bags (e.g., rye, millet, or sawdust) before inoculating.
- Shake the syringe gently to evenly distribute the mycelium in the liquid.
- Draw the liquid culture into the syringe (usually 1-2ml per jar or bag).
- Insert the needle through the sterilized lid or filter patch of your jar/bag, and inject the culture into the substrate. If you're using jars, consider placing the needle just below the surface to allow even distribution.
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Post-Inoculation Care:
- Store your inoculated jars or bags in a warm (70-80°F / 21-27°C) and dark location to encourage mycelial growth.
- Ensure your jars/bags have proper air exchange to allow the mycelium to breathe (especially if using bags with filter patches).
- Check your jars regularly for signs of contamination (off-color growth or foul smell) and for full colonization of the substrate. Once colonized, your mycelium is ready for fruiting!
Storage Instructions:
- Short-Term: Store your liquid culture syringes in the refrigerator (36-40°F / 2-4°C) until you're ready to use them. This will help preserve the mycelium and prevent premature growth.
- Long-Term: For long-term storage, the syringes can remain in the fridge for up to 6 months. However, it’s recommended to use them within 1-3 months for the best results.
Tips for Success:
- Use a clean needle for each inoculation to avoid cross-contamination.
- For larger inoculations, you can shake the syringe to disperse mycelium into multiple jars or bags.
- If you’re having trouble with slow colonization, ensure that your substrate is properly sterilized and that the environment is warm and clean.
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